Search results for "Hairy root culture"

showing 4 items of 4 documents

Genetic Transformation of Serratula tinctoria (Dyer’s Savory) for Ecdysteroid Production

1999

Serratula tinctoria is a perennial plant of the Compositae family with medium-sized, serrated leaves and purple flowers (Loste 1937). This plant, also known as dyer’s savory, is widespread in Europe but with an irregular distribution. Inflorescences (capitula) are purple and are usually unisexual, staminate, or pistillate. In Europe, the flowering period extends from July to September. More than 40 species have been described in Europe, North Africa, and Asia. The plants produce large amounts of secondary metabolites, in particular ecdysteroids at very high concentration in roots (up to 2% dry wt.), in flowers, and in leaves (Bathori et al. 1986; Rudel et al. 1992; Corio-Costet et al. 1993b…

0106 biological sciencesHigh concentration0303 health sciencesEcdysteroidPolypodine BbiologyPerennial plant[SDV]Life Sciences [q-bio]Stamenbiology.organism_classification01 natural sciences03 medical and health scienceschemistry.chemical_compoundSerratulaInflorescencechemistryBotanyHairy root cultureComputingMilieux_MISCELLANEOUS030304 developmental biology010606 plant biology & botany
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Serratula tinctoria (L.) (Dyer's savory): In vitro culture and the production of ecdysteroids and other secondary metabolites

1996

The name Serratula is derived from the Latin word serra, which means saw. It designates perennial plants of the Compositae family with medium-sized, serrated leaves and purple flowers (Loste 1937). More than 40 species have been described in Europe, North Africa, and Asia. The most common is Serratula tinctoria, also known as dyer’s savory. This species, 30 to 80 cm high, grows on all kind of soils, and is widely distributed in central and northern Europe (synonym: Carduus tinctoria, Scop; French name: serratule des teinturiers; German name: Faberscharte). In France, for example, Serratula tinctoria is widespread but with an irregular distribution, rare in the north and near the Mediterrane…

0106 biological sciencesMediterranean climate0303 health sciencesPerennial plantEcology[SDV]Life Sciences [q-bio]North africaLatin word15. Life on landBiologybiology.organism_classification01 natural sciences[SDV] Life Sciences [q-bio]03 medical and health sciencesSynonym (taxonomy)SerratulaHairy root cultureBotanyCarduusComputingMilieux_MISCELLANEOUS030304 developmental biology010606 plant biology & botany
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Isolation and Structure Elucidation of a New Indole Alkaloid from Rauvolfia serpentina Hairy Root Culture: The First Naturally Occurring Alkaloid of …

2002

A new monoterpenoid indole alkaloid, 10-hydroxy- N(alpha)-demethyl-19,20-dehydroraumacline ( 1), was isolated as a mixture of E- and Z-isomers from hairy root culture of Rauvolfia serpentina Benth. ex Kurz (Apocynaceae) and the structure was determined by 1D and 2D NMR analyses. The new indole alkaloid represents the first naturally occurring alkaloid of the raumacline group and its putative biosynthetical pathway is discussed.

Magnetic Resonance SpectroscopyStereochemistryMonoterpenePharmaceutical SciencePharmacognosyPlant RootsRauwolfiaIndole AlkaloidsAnalytical ChemistryRauvolfia serpentinaDrug DiscoveryBotanyCells CulturedPharmacologyCarbon IsotopesMolecular StructurebiologyApocynaceaeIndole alkaloidPlant ExtractsAlkaloidOrganic ChemistryStereoisomerismbiology.organism_classificationSecologanin Tryptamine AlkaloidsTerpenoidComplementary and alternative medicineHairy root cultureMolecular MedicinePlanta Medica
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New Alkaloids of the Sarpagine Group from Rauvolfia serpentina Hairy Root Culture

2002

Three new monoterpenoid indole alkaloids, 19(S),20(R)-dihydroperaksine (1), 19(S),20(R)-dihydroperaksine-17-al (2), and 10-hydroxy-19(S),20(R)-dihydroperaksine (3), along with 16 known alkaloids 4-19 were isolated from hairy root culture of Rauvolfia serpentina, and their structures were elucidated by 1D and 2D NMR analyses. Taking into account the stereochemistry of the new alkaloids and results of preliminary enzymatical studies, the putative biosynthetical relationships between the novel alkaloids are discussed.

Monoterpenoid Indole AlkaloidsStereochemistryPharmaceutical SciencePharmacognosyPlant RootsRauwolfiaIndole AlkaloidsAnalytical ChemistryRauvolfia serpentinaDrug DiscoveryNuclear Magnetic Resonance BiomolecularPharmacologyFolk medicinePlants MedicinalMolecular StructurebiologyApocynaceaeChemistryAlkaloidOrganic ChemistryStereoisomerismbiology.organism_classificationSecologanin Tryptamine AlkaloidsTerpenoidComplementary and alternative medicineHairy root cultureMolecular MedicineChromatography Thin LayerRhizobiumJournal of Natural Products
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